Method of recovering highly purified vWF or factor VIII/vWF-complex

US6579723B1 · kind B1 · utility

Assignee

• Baxter Aktiengesellschaft · AT

Inventors

• Artur Mitterer · Orth an der Donau, AT
• Christian Fiedler · Wien, AT
• Bernhard Fischer · Wien, AT
• Friedrich Dorner · Wien, AT
• Johann Eibl · Wien, AT

Key dates

• Filing date: Oct 21, 1999
• Grant date: Jun 17, 2003
• Expiry date: Oct 21, 2019

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method for purifying factor VIII/vWF complex or free vWF by immunoaffinity chromatography in a form suitable for use as a medicament. Factor VIII/vWF complex or free vWF is recovered from an immunoaffinity adsorbent by using an eluting agent containing a zwitterionic species. The presence of the zwitterionic species allows for the use of mild conditions throughout the preparation, facilitating retention of molecular integrity, activity, and incorporation of the recovered proteins into pharmaceutical preparations without the need for additional stabilizers or preservatives.