Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6582914B1 · kind B1 · utility
58Cited by
6References
10Claims
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Key dates
| Filing date | Oct 26, 2000 |
| Grant date | Jun 24, 2003 |
| Priority date | — |
| Expiry date | Oct 26, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are disclosed for producing libraries of nucleic acid molecules which libraries are derived from a nucleic acid template. The libraries comprise variant nucleic acids which are produced from a mutagenesis strategy using, e.g., a plurality of defined mutagenic and/or non-mutagenic primers and specific reaction conditions which favor the production of varied combinatorial mutants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.