Method for detecting protein-protein interactions and a kit therefor
US6582927B2 · kind B2 · utility
1Cited by
1References
9Claims
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Key dates
| Filing date | Jan 22, 2001 |
| Grant date | Jun 24, 2003 |
| Priority date | — |
| Expiry date | Jan 22, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for detecting an interaction between two tester proteins. In a cell incapable of activating a Ras protein, two nucleic acid sequences are expressed. One sequence encodes for a fusion protein comprising a mutant Ras protein incapable of localizing at the cell membrane and not requiring an exchange factor fused to one of the tester proteins. The other sequence encodes for the other tester protein fused to a plasma membrane localization domain. An interaction between the two fusion proteins leads to the expression of a functional Ras protein that is tested as an altered cell phenotype.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.