Gel microdrops in genetic analysis
US6586176B1 · kind B1 · utility
263Cited by
6References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 6, 1999 |
| Grant date | Jul 1, 2003 |
| Priority date | — |
| Expiry date | Aug 6, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of nucleic acid analysis. Such methods entail forming a population of gel microdrops encapsulating a population of biological entities, each entity comprising a nucleic acid, whereby at least some microdrops in the population each encapsulate a single entity. The population of gel microdrops is then contacted with a probe under conditions whereby the probe specifically hybridizes to at least one complementary sequence in the nucleic acid in at least one gel microdrop. At least one gel microdrop is then analyzed or detected. The biological entities can be cells, viruses, nuclei and chromosomes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.