Multiple enzyme assays
US6586196B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 14, 1999 |
| Grant date | Jul 1, 2003 |
| Priority date | — |
| Expiry date | Dec 14, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/95
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a cell extract, wherein at least one of the enzymes is an endogenous enzyme. In one embodiment of the invention the activity of a first enzyme is quantified by measuring the light signal produced by degradation of a first enzyme substrate by the first enzyme and the activity of the second enzyme is quantified by measuring the light signal produced by the degradation of a second substrate. In the method of the present invention, both quantifications are performed on the same aliquot of cell extract. Different embodiments of the present invention provide for the detection of more than one endogenous enzyme and for the detection of at least one reporter enzyme and at least one endogenous enzyme. The present invention also discloses kits for detecting the activity of multiple enzymes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.