Method for detecting substances inhibiting the bacterial type III secretion mechanism and function of secretory proteins thereof
US6586200B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 21, 2002 |
| Grant date | Jul 1, 2003 |
| Priority date | — |
| Expiry date | May 21, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/255
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method for detecting substances specifically inhibiting a type III secretion mechanism and functions of the type III secretory proteins, within short time and large amounts thereof, without depending upon animal infectious experiments. Namely it relates to the method for detection of a type III secretory mechanism inhibitor comprising mixing a bacterium having the type III secretory mechanism and an erythrocyte suspension, adding the type III secretory mechanism inhibitor thereto, and detecting changes in the thus formed hemolytic activity. The method for detecting substances can be treated large amount of samples within short time by exhibiting the substances inhibiting the type III secretion mechanism or the functions of the type III secretory proteins as numerical index of the hemolytic activity of erythrocytes. Consequently, the present invention is useful for development of drugs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.