Patent · US Expired

Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions

US6586218B2 · kind B2 · utility

34Cited by

1References

26Claims

0Family size

Assignee

Ambion, Inc. · US

Inventors

Susan Milburn · Austin, US
Marianna Goldrick · Pflugerville, US
Matthew Winkler · Austin, US

Key dates

Filing date	Sep 28, 2001
Grant date	Jul 1, 2003
Priority date	—
Expiry date	Sep 28, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P19/34
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA trancription and in various other enzymatic reactions in which a polynucleotide is synthesised. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.