Method for more efficient electroporation
US6586249B2 · kind B2 · utility
1Cited by
5References
36Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 15, 2002 |
| Grant date | Jul 1, 2003 |
| Priority date | — |
| Expiry date | Jan 15, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/64
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.