Microchip based enzymatic analysis
US6586253B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 10, 1998 |
| Grant date | Jul 1, 2003 |
| Priority date | — |
| Expiry date | Apr 10, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the detection of cell contents, the method comprising the steps of, introducing a cell into a channel in a microchip; lysing the cell to release cell contents into the channel; moving the cell contents towards a detection zone; and detecting the cell contents at the detection zone. An apparatus for the detection of cell contents, the apparatus comprising: a microchip; a cell mobilization channel formed in the microchip, the cell mobilization channel having a cell introduction end and a detection end; a cell mobilizer operably connected with the cell introduction end for moving cells from the cell introduction end to the detection end; means for lysing cells in the cell mobilization channel at a lysing zone, the lysing zone being located between the cell introduction end and the detection end; and a detector, disposed adjacent the detector end, arranged to detect cell contents appearing at the detector end that have been moved from the lysing zone to the detector end by the cell mobilizer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.