Method and device for detecting hybridization reaction
US6589740B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 9, 2001 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Mar 9, 2021 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0819
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention detects and quantities only specific hybridization bindings. A biochip 10 which is spotted with a plurality of probe biopolymers is accommodated in a container 20 into which a washing solution is supplied from a liquid supplying unit 23. A heat block 31 controls the temperature of the biochip according to a predetermined time pattern. A pickup unit 55 picks up an image of the spot surface of the biochip at predetermined pickup intervals. The plurality of images picked up with the pickup unit are stored in a computer 40. By analyzing the images for individual spots, hybridization can be detected with high reliability for every spots without being influenced by optimal hybridization temperatures which differ depending upon the types of probes on the spots.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.