Polysome display in the absence of functional ssrA-RNA
US6589741B2 · kind B2 · utility
21Cited by
4References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 14, 2001 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Sep 14, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K16/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules. The method of the present invention is particularly characterized by an in vitro translation step under conditions that allow formation of polysomes in the presence of antisense oligonucleotides complementary to the tag-coding sequence of ssrA-RNA. The present invention further relates to kits that are useful for carrying out the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.