Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6589742B2 · kind B2 · utility
7Cited by
31References
47Claims
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Key dates
| Filing date | Sep 17, 2001 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Sep 17, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/607
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for amplifying nucleic acids is provided wherein detection of amplified species is enhanced by the use of asymmetric amplification. Such amplification is made asymmetric by using divergent ratios of amplification primers or by using non-extending and/or non-cleavable amplification primers. Detection of the amplicons is improved because maintenance of single stranded species of amplicons during amplification facilitates their direct capture by immobilized probes without having to include denaturing steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.