Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof
US6589744B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 2001 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Nov 26, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and kit for detecting nucleic acid modification enzyme activity by monitoring the change in fluorescence polarization upon the change in mass of a fluorescently labeled oligo. A nucleic acid substrate comprises a template having a nucleic acid sequence annealed to a labeled oligo, wherein there is a nick or break located between the adjacent ends of the template and the labeled oligo. A nucleic acid modification enzyme is added to the substrate to form a reaction mixture. A stop reagent may or may not be used to further process the reaction mixture. A test compound is added to the reaction mixture, preferably prior to addition of the nucleic acid modification enzyme, whereby the resulting fluorescence polarization signal is compared to a standard signal to determine if the test compound inhibits nucleic acid modification enzyme activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.