Methods for arousing dormant bacteria
US6589771B1 · kind B1 · utility
31Cited by
1References
3Claims
0Family size
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Inventor
Key dates
| Filing date | Oct 28, 1999 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Oct 28, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/875
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for arousing dormant bacteria. The method comprises inducing diffusion of intracellular solutes from dormant bacteria and then allowing an adjustment period for a length of time sufficient to initiate arousal. The decrease in intracellular osmolality or pH can be induced by methods such as extraction, dilution, or dialysis. The method has been standardized using Dulbecco's phosphate buffered saline as the solution. The aroused bacteria can then be selected or recovered by growing them on media for a period of time. If the adjustment period is prolonged, many bacteria can become hypermutative.
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