Multiple host expression vector
US6589783B2 · kind B2 · utility
8Cited by
1References
9Claims
0Family size
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Key dates
| Filing date | Apr 13, 2000 |
| Grant date | Jul 8, 2003 |
| Priority date | — |
| Expiry date | Apr 13, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14143
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A triple host vector is described which is capable of expressing an inserted protein coding DNA sequence in any of three distinct host systems. The vector includes three promoters, one each for vertebrate, bacterial and insect host cells, so that the protein coding sequence can be cloned into the vector once and the vector can be used to express protein in all three types of host cells. The arrangement and selection of the promoters contributes to expression characteristics in all three host systems that is comparable to single host expression vectors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.