Disrupting cells and/or tissue in solid state in presence of solid denaturing substance

US6589785B1 · kind B1 · utility

Assignee

• Aventis Research & Technologies GmbH & Co. · DE

Inventors

• Stefan Mullner · Langenfeld, DE
• Thomas Neumann · Munich, DE

Key dates

• Filing date: Jul 31, 2000
• Grant date: Jul 8, 2003
• Expiry date: Jul 31, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N1/06
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for disrupting frozen cells and/or tissue in a solid, frozen state in the presence of a solid denaturing substance is provided. The method comprises providing frozen cells and/or tissue in frozen solid form, adding thereto the solid denaturing substance in sold form, and applying mechanical force thereto for disruption of the frozen cells and/or tissue. The frozen cells and/or tissue include muscle cells and/or muscle tissue, wheat cells and/or wheat tissue, and maize cells and/or maize tissue. Further, the solid, denaturing substance in solid form is a crystalline substance and can be selected from urea, thiourea, guanidium chloride, guanidium thiocyanate and ammonium sulfate. Also the solid, denaturing substance in solid form is added to the frozen cells and/or tissue in frozen form in an approximately 1 to 20 fold (w/w) excess.