Directional assembly of large viral genomes and chromosomes
US6593111B2 · kind B2 · utility
20Cited by
2References
53Claims
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Key dates
| Filing date | May 21, 2001 |
| Grant date | Jul 15, 2003 |
| Priority date | — |
| Expiry date | May 21, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/20043
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Full-length, functionally intact genomes or chromosomes are directionally assembled with partial cDNA or DNA subclones of a genome. This approach facilitates the reconstruction of genomes and chromosomes in vitro for reintroduction into a living host, and allows the selected mutagenesis and genetic manipulation of sequences in vitro prior to reassembly into a full length genome molecule for reintroduction into the same or different host. This approach also provides an alternative to recombination-mediated techniques to manipulate the genomes of higher plants and animals as well as bacteria and viruses.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.