Modified MSP-1 nucleic acid sequences and methods for increasing MRNA levels and protein expression in cell systems
US6593463B1 · kind B1 · utility
10Cited by
3References
22Claims
0Family size
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Key dates
| Filing date | Oct 20, 1998 |
| Grant date | Jul 15, 2003 |
| Priority date | — |
| Expiry date | Oct 20, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides modified recombinant nucleic acid sequences (preferably DNA) and methods for increasing the mRNA levels and protein expression of malarial surface protein MSP-1 which is known to be difficult to express in cell culture systems, mammalian cell culture systems, or in transgenic animals. The preferred protein candidates for expression using the recombinant techniques of the invention are MSP-1 proteins expressed from DNA coding sequences comprising reduced overall AT content or AT rich regions and/or mRNA instability motifs and/or rare codons relative to the native MSP-1 gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.