Recombinant constructs and systems for secretion of proteins via type III secretion systems
US6596509B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 1999 |
| Grant date | Jul 22, 2003 |
| Priority date | — |
| Expiry date | Jul 9, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/43
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
One aspect of the present invention relates to a DNA construct that contains a first DNA molecule encoding a functional type III secretion system, a promoter, and a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. The second DNA molecule is operably coupled to the promoter so that upon introduction of the DNA construct into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Another aspect of the present invention relates to a system that includes (i) a first DNA construct having a first DNA molecule encoding a functional type III secretion system and (ii) a second DNA construct having a promoter operably coupled to a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. Upon introduction of the first and second DNA constructs into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Methods of isolating a protein or polypeptide and identifying a gene encoding a potential…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.