Patent · US Expired

Process for the fermentative preparation of L-amino acids using coryneform bacteria

US6596516B2 · kind B2 · utility

1Cited by

0References

12Claims

0Family size

Assignees

Degussa AG · DE
Forschungszentrum Jülich GmbH · DE

Inventors

Petra Ziegler · Aachen, DE
Lothar Eggeling · Jülich, DE
Hermann Sahm · Jülich, DE
Georg Thierbach · Bielefeld, DE
Walter Pfefferle · Bodenwerder, DE

Key dates

Filing date	Dec 8, 2000
Grant date	Jul 22, 2003
Priority date	—
Expiry date	Feb 12, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P13/08
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A process for the preparation of L-amino acids, in which the following steps are carried out,a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionallyb) concentrating the desired product in the medium or in the cells of the bacteria andc) isolating the L-amino acid,and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.