Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli
US6596524B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Sep 20, 2001 |
| Grant date | Jul 22, 2003 |
| Priority date | — |
| Expiry date | Nov 14, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in E. coli cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.