Rapid immunoassay to detect infection with Mycobacterium tuberculosis

US6599691B1 · kind B1 · utility

Assignee

• United States of America as represented by the Secretary of the Air Force · US

Inventors

• Stephen Alden Ralls · Great Lakes, US
• Lloyd G. Simonson · Spring Grove, US

Key dates

• Filing date: Feb 24, 1997
• Grant date: Jul 29, 2003
• Expiry date: Dec 9, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5695
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A rapid, non-invasive, semi-quantitative immunoassay of saliva has been developed to aid in the diagnosis of diseases, e.g., using saliva to detect subjects actively or previously infected with Mycobacterium tuberculosis, a causative organism of tuberculosis. The semi-quantitative assay comprises spotting disease-related antigens on the surface of a solid substrate; contacting the solid substrate with a saliva sample which, in positive subjects, contains primary antibodies to the disease-related antigens; contacting the primary antibodies with a label capable of being detected; and detecting and reading the label whereby exposure to the antigens is determined. The device for conducting these assays is a frame or support which holds a solid substrate capable of immobilizing the antigens of interest while permitting drainage of other materials or fluids away from the immobilized antigens. A less rapid, quantitative assay has also been developed by adapting the rapid, semi-quantitative assay to an enzyme linked immunosorbant assay thereby providing a quantitative assay capable of assessing multiple saliva samples simultaneously.