Method to isolate mutants and to clone the complementing gene
US6599745B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 11, 2000 |
| Grant date | Jul 29, 2003 |
| Priority date | — |
| Expiry date | Jul 11, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01099
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.