Process for separating and analyzing hydrophobic proteins using thin layer chromatography

US6599752B1 · kind B1 · utility

Assignee

• ALTANA PHARMA AG · DE

Inventors

• Wolfgang Ise · Konstanz, DE
• Rüdiger Nave · Konstanz, DE
• Wolfram Steinhilber · Stockach, DE

Key dates

• Filing date: Nov 13, 1998
• Grant date: Jul 29, 2003
• Expiry date: Dec 8, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/785
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This-layer chromatography is used to separate, identify and quantity hydrophobic protein, hydrophobic protein fragment, hydrophobic modified protein and hydrophobic peptide. A method is thus provided for determination of proteins and peptides which, due to their low solubility in an aqueous solvent system, cannot be determined by conventional methods, such as ELISA, which are based on aqueous solvent systems.