Thermostable luciferases and methods of production
US6602677B1 · kind B1 · utility
127Cited by
5References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 15, 1999 |
| Grant date | Aug 5, 2003 |
| Priority date | — |
| Expiry date | Sep 15, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/898
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.