Phenolic acid esterases, coding sequences and methods
US6602700B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 21, 2000 |
| Grant date | Aug 5, 2003 |
| Priority date | — |
| Expiry date | Jun 21, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/18
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a feruloyl esterase of Ruminococcus. The fourth specifically exemplified phenolic acid esterase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided. Further provided are methods for improving nutrient availability and ferulic acid availability when food or feed, or other material is treated with a phenolic acid esterase, desirably in combination with a xylanase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.