Gene functional analysis and discovery using randomized or target-specific ribozyme gene vector libraries
US6605429B1 · kind B1 · utility
5Cited by
36References
6Claims
0Family size
Assignee
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Key dates
| Filing date | Jul 23, 1999 |
| Grant date | Aug 12, 2003 |
| Priority date | — |
| Expiry date | Jul 23, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2799/025
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a hairpin ribozyme library having a randomized recognition sequence, packaged in a vector and operably linked to a promoter suitable for high level expression in a wide variety of cells. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.