DNA shuffling of monooxygenase genes for production of industrial chemicals
US6605430B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 12, 1999 |
| Grant date | Aug 12, 2003 |
| Priority date | — |
| Expiry date | Aug 12, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1086
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides improved monoxygenases, dehydrogenases, and transferases that are useful for the biocatalytic synthesis of compounds such as &agr;-hydroxycarboxylic acids, and aryl- and alkyl-hydroxy compounds. The polypeptides provided herein are improved in properties such as regioselectivity, enzymatic activity, stereospecificity, and the like. Methods for obtaining recombinant polynucleotides that encode these improved polypeptides are also provided, as are organisms that express the polypeptides and are thus useful for carrying out said biocatalytic syntheses. Also provided by the invention are methods for increasing said solvent resistance of organisms that are used in the synthetic methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.