Extraction, amplification and sequential hybridization of fungal cell DNA and process for detection of fungal cells in clinical material
US6605439B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | May 29, 2001 |
| Grant date | Aug 12, 2003 |
| Priority date | — |
| Expiry date | May 29, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6895
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
For the detection of fungal cells in clinical material fungal DNA is extracted from whole blood and the extracted fungal DNA is then detected. From this detection, a fungal infection can be concluded. For further diagnosis, the fungal species are then determined from the extracted fungal DNA. The method for extraction of the fungal DNA from whole blood comprises the isolation of predominantly intact fungal cells from the whole blood and the extraction of DNA from the isolated fungal cells. For detection of fungal DNA, at least one segment of the fungal DNA to be detected is amplified, then the amplification products are detected. For further identification of the fungal species, nucleotide sequence segments being characteristic for the fungal species are detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.