Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of a said peptide and a heterologous protein
US6605697B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 7, 2001 |
| Grant date | Aug 12, 2003 |
| Priority date | — |
| Expiry date | Mar 7, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified E. coli enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, the modified E. coli enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th, and 22nd amino acids of E. coli enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.