Polymerase chimeras

US6607883B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Bruno Frey · Penzberg, DE
• Britta Villbrandt · Braunschweig, DE
• Dietmar Schomburg · Braunschweig, DE
• Harald Sobek · Penzberg, DE
• Waltraud Ankenbauer · Penzberg, DE

Key dates

• Filing date: Feb 8, 2001
• Grant date: Aug 19, 2003
• Expiry date: Feb 8, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.