Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay
US6607906B1 · kind B1 · utility
0Cited by
8References
70Claims
0Family size
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Key dates
| Filing date | Apr 9, 1998 |
| Grant date | Aug 19, 2003 |
| Priority date | — |
| Expiry date | Apr 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A first aspect of the present invention is directed to a transformed yeast cell containing a first heterologous DNA sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second heterologous DNA sequence which codes for a G protein a subunit or portions thereof fused to DNA sequences from the yeast G protein in a subunit. A second aspect of the present invention is a transformed yeast cell containing a heterologous DNA sequence which codes for a G protein coupled receptor. A third aspect of the present invention is a method of assaying compounds to determine effects on cell growth.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.