Patent · US Expired

Compositions, kits and uses of nucleotides having altered base pairing characteristics

US6610490B2 · kind B2 · utility

14Cited by
4References
39Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 28, 2001
Grant dateAug 26, 2003
Priority date—
Expiry dateJun 28, 2021

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/156
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention provides improved methods, compositions and kits for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with nucleotide residue having altered base-pairing characteristics, such as inosine, hypoxanthine, xanthine, and methylated nucleotide derivatives, so as to more equalize the efficiency with which that oligonucleotide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.