Cold-shock regulatory elements, constructs thereof, and methods of use

US6610533B1 · kind B1 · utility

Assignee

• University of Medicine and Dentistry of New Jersey · US

Inventors

• Masayori Inouye · Bound Brook, US
• Nan Wang · Shanghai, CN
• Kunitoshi Yamanaka · Ooita, JP

Key dates

• Filing date: Mar 1, 2000
• Grant date: Aug 26, 2003
• Expiry date: Mar 1, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/245
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A fourth cold shock protein of the Escherichia coli CspA family is disclosed, as are the regulatory elements of the 5′ UTR of the corresponding gene. The cspI gene is located at 35.2 min on the E. coli chromosome map, and CspI shows 70, 70, and 79% identity of CspA, CspB, and CspG, respectively. The 5′-untranslated region of the cspI mRNA consists of 145 bases and causes a negative effect on cspI expression at 37° C. The cspI mRNA was very unstable at 37° C. but was stabilized upon cold shock. The 5′ UTR of cspI can enhance the translation of cold shock inducible genes under conditions that elicit a cold shock response in bacteria.