Sequencing by incorporation

US6613513B1 · kind B1 · utility

Assignee

• Caliper Technologies, Corp. · US

Inventors

• J. Wallace Parce · Palo Alto, US
• Theo Nikiforov · Carlsbad, US
• Tammy Burd Mehta · San Jose, US
• Anne R. Kopf-Sill · Portola Valley, US
• Andrea W. Chow · Los Altos, US
• Michael R. Knapp · Redwood City, US

Key dates

• Filing date: Feb 22, 2000
• Grant date: Sep 2, 2003
• Expiry date: Feb 22, 2020

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.