Determination of a specific immunoglobulin using multiple antigens

US6613530B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Ursula-Henrike Wienhues · Krailling, DE
• Cornelia Kruse-Müller · Tutzing, DE
• Eva Höss · Starnberg, DE
• Elke Faatz · Huglfing, DE
• Beatus Ofenloch-Hahnle · Wielenbach, DE
• Christoph Seidel · Weilheim, DE
• Michael Wiedmann · Penzberg, DE

Key dates

• Filing date: Jan 24, 1997
• Grant date: Sep 2, 2003
• Expiry date: Jan 24, 2017

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/811
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns a method for the immunological determination of a specific antibody in a sample liquid in which the sample liquid is incubated in the presence of a solid phase with two antigens directed against the antibody to be determined of which the first antigen carries at least one marker group and the second antigen is (a) bound to the solid phase or (b) is present in a form capable of binding to the solid phase and the antibody to be determined is detected by determining the marker group in the solid phase or/and in the liquid phase characterized in that at least one of the two antigens contains several epitope regions which react with the antibody to be determined.