Nucleotide sequences coding for the export of branched chain amino acids, process for the isolation thereof and use thereof

US6613545B1 · kind B1 · utility

Assignees

• Degussa-Huels AG · DE
• Forschungszentrum Jülich GmbH · DE

Inventors

• Nicole Kennerknecht · Krefeld, DE
• Hermann Sahm · Jülich, DE
• Lothar Eggeling · Jülich, DE
• Walter Pfefferle · Bodenwerder, DE

Key dates

• Filing date: Dec 23, 1999
• Grant date: Sep 2, 2003
• Expiry date: Dec 23, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/08
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the groupa) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing at least one amino acid sequence of SEQ ID no. 3 or 5,b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 3 or 5,c) polynucleotide which is complementary to the polynucleotides of a), b) or c), andd) polynucleotide containing at least 15 successive bases of the polynucleotide sequences of a), b) or c).wherein the polypeptides exhibit the biological activity of the enzymes for which the brnE or bernF (sic) gene codes and a process for the fermentative production of branched-chain L-amino acids with amplification of the stated genes.