Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US6617108B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 3, 2000 |
| Grant date | Sep 9, 2003 |
| Priority date | — |
| Expiry date | Nov 20, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Preferred embodiments of the invention include purification of DNA, preferably plasmid DNA, by use of selective precipitation, preferably by addition of compaction agents. Also, included is a sealable method for the liquid phase separation of DNA from RNA. RNA may also be recovered by fractional precipitation according to the invention. Applicants have discovered that RNA, commonly the major contaminant in DNA preparations, can be left in solution while valuable purified plasmid DNA is directly precipitated. Additional aspects of the invention include mini-preps, preferably of plasmid and chromosomal DNA, to obtain sequenceable and restriction digestible DNA in high yields in multiple simultaneous procedures. Still further aspects disclose enhanced stripping of the compaction agent by a stripping method comprising high salt addition and pH shift, and combinations of these techniques. Also, disclosed is a method of assay in which a labeled probe is precipitated when it is hybridized to a target, (e.g. chromosomal DNA, oligonuclotides, Ribosomal RNA, tRNA), and thereafter precipitating the probe/target complex with compaction agents and leaving in solution any unhybridized probe. For…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.