High numerical aperture flow cytometer and method of using same
US6618143B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 2, 2001 |
| Grant date | Sep 9, 2003 |
| Priority date | — |
| Expiry date | Oct 2, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/4704
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The high numerical aperture flow cytometer of the present invention includes a flow cell and a laser input. The laser input emits a beam of light that is oriented substantially orthogonally to the flow of blood cells through the flow cell such that laser light impinges upon the blood cells as they pass through the flow cell. A portion of the beam from the laser input that impinges upon the blood cells in the flow cell is scattered at a substantially right angle to the beam of laser input (“right angle scatter”). A second portion of the beam from the laser input that impinges upon the cells in the flow cell is scattered at a much lower angle than 90°. This scatter is termed forward scatter light” and is collected on two distinct photo detectors, that represent ‘forward scatter low’ (FSL) which has an angle of from about 1° to about 3°, and ‘forward scatter high’ (FSH) which has an angle of from about 9° to about 12° from the orientation of the original beam from laser input. A third photo detector is placed in between these two forward scatter detectors, that is axial with the impinging laser light. This detector measures…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.