Nucleotide sequences encoding the sucC and sucD genes

US6623946B1 · kind B1 · utility

Assignee

• Degussa AG · DE

Inventors

• Bettina Mockel · Bielefeld, DE
• Walter Pfefferle · Bodenwerder, DE
• Achim Marx · Bielefeld, DE

Key dates

• Filing date: Nov 27, 2000
• Grant date: Sep 23, 2003
• Expiry date: Apr 16, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/14
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Polynucleotides that contain polynucleotide sequences encoding the sucC and sucD genes, selected from the groupa) a polynucleotide that is at least 70% identical to a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID NO:2,b) a polynucleotide that is at least 70% identical to a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID NO:3,c) a polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO:2,d) a polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO:3,e) a polynucleotide that is complementary to one of the polynucleotides of a), b), c) or d), andf) a polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b), c), d) or e),a process for the fermentative production of L-amino acids using coryneform bacteria in which the genes are present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.