System for reproducing and modulating stability and turnover of RNA molecules
US6627398B1 · kind B1 · utility
16Cited by
2References
45Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 26, 1999 |
| Grant date | Sep 30, 2003 |
| Priority date | — |
| Expiry date | May 26, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1037
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
An in vitro system is provided that recapitulates regulated mRNA stability and turnover of exogenous RNA substrates. The system comprises a cell extract optionally depleted of activity of proteins that bind polyadenylate, and a target RNA sequence. This system is used for the identification of agents capable of modulating RNA turnover, as well as agents capable of modulating RNA turnover in the presence of RNA stability modifying agents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.