5′-modified nucleotides and the application thereof in molecular biology and medicine

US6627416B1 · kind B1 · utility

Assignee

• Europaeisches Laboratorium Fuer Molekularbiologie (EMBL) · DE

Inventors

• Konrad Faulstich · Fremont, US
• Wilhelm Ansorge · Gaiberg, DE

Key dates

• Filing date: Jan 10, 2001
• Grant date: Sep 30, 2003
• Expiry date: Jan 10, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to 5′-modified nucleotides and to nucleic acids which contain these nucleotides. Processes for incorporating the 5′-modified nucleotides into nucleic acids, and the subsequent site-specific cleavage of the nucleic acids at the 5′-modified monomer building blocks, are also disclosed. These processes can be employed for nucleic acid sequencing, for generating nucleic acid libraries, for detecting mutations, for preparing support-bound nucleic acids and for pharmaceutical purposes.