Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses

US6627748B1 · kind B1 · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Jingyue Ju · Englewood Cliffs, US
• Zengmin Li · Great Neck, US
• Anthony Tong · New York, US
• James J. Russo · New York, US

Key dates

• Filing date: Sep 11, 2000
• Grant date: Sep 30, 2003
• Expiry date: Oct 18, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescence emission signature. The invention further provides for the use of said tags in multi-component analyses, including multiplex genetic analyses.