Methods for obtaining, identifying and applying nucleic acid sequences and (poly)peptides which increase the expression yields of periplasmic proteins in functional form
US6630317B1 · kind B1 · utility
1Cited by
1References
12Claims
0Family size
Assignee
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Key dates
| Filing date | May 1, 2000 |
| Grant date | Oct 7, 2003 |
| Priority date | — |
| Expiry date | May 1, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for obtaining nucleic acid sequences encoding (poly)peptides which increase the expression yields of periplasmic proteins in functional form upon co-expression of said (poly)peptides and said periplasmic proteins. The invention also provides a method for the identification of said (poly)peptides. Furthermore, the present invention relates to a method for increasing the expression yields of periplasmic proteins in functional form by co-expressing (poly)peptides, for example Skp, FkpA, or a homolog of Skp or FkpA, in bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.