Herbicides test method

US6630331B1 · kind B1 · utility

Assignee

• Hoechst Schering Agrevo GmbH · DE

Inventors

• Christopher Abell · Cambridge, GB
• Alison Gail Smith · Chippenham, GB
• Ulrich Genschel · Hamburg, DE
• Bernd Laber · Idstein, DE

Key dates

• Filing date: Nov 23, 1999
• Grant date: Oct 7, 2003
• Expiry date: Nov 23, 2019

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/573
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An isolated DNA molecule encoding a protein from a plant, which protein has pantothenate synthetase activity; a non-naturally occuring chimeric gene comprising a promoter operably linked to a DNA molecule encoding a protein from a plant having pantothenate synthetase activity; a recombinant vector comprising the chimeric gene wherein the vector is capable of being stably transformed into host cell, a host cell stably transformed with a vector wherein the host cell is capable of expressing the DNA molecule; a method for assaying a protein having pantothenate synthetase activity; the use as herbicides of compounds which inhibit pantothenate synthetase, and a herbicidal composition, comprising one or more active ingredients which show significant pantothenate synthetase inhibition in an assay, are disclosed.The invention relates to plant enzymatic activity, and aspects therefor, involved in the biosynthesis of coenzyme A. The invention particularly relates to the plant enzyme known either as pantoate B-alanine ligase, pantoate activating enzyme or pantothenate synthetase (PS).