Method of target enrichment and amplification
US6632611B2 · kind B2 · utility
64Cited by
12References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 20, 2001 |
| Grant date | Oct 14, 2003 |
| Priority date | — |
| Expiry date | Jul 20, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The presently claimed invention provides methods and kits for amplifying a target sequence from within a nucleic acid population. The presently claimed invention provides selection probes which are complementary to at least a portion of said target sequence and mechanisms for adding a probe sequence to the 3′ end of a target sequence that is hybridized to a selection probe. The added 3′ probe sequence and a probe sequence added at the 5′ end of the target by adaptor ligation allow for selective amplification of the target sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.