Method for isolating a target biological material, capture phase, detecting phase and reagent containing them
US6632615B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 1999 |
| Grant date | Oct 14, 2003 |
| Priority date | — |
| Expiry date | Apr 21, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognizing or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues, the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.