Patent · US Expired

Microfabricated apparatus for cell based assays

US6632656B1 · kind B1 · utility

107Cited by
14References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 17, 2000
Grant dateOct 14, 2003
Priority date
Expiry dateNov 17, 2020

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/0688
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

Apparatus and methods are provided for performing cell growth and cell based assays in a liquid medium. The apparatus comprises a base plate supporting a plurality of micro-channel elements, each micro-channel element comprising a cell growth chamber, an inlet channel for supplying liquid sample thereto and an outlet channel for removal of liquid sample therefrom, a cover plate positioned over the base plate to define the chambers and connecting channels, the cover plate being supplied with holes to provide access to the channels. Means are incorporated in the cell growth chambers, for cell attachment and cell growth. In particular, the invention provides a rotatable disc microfabricated for performing cell growth and cell based assays. The apparatus and method can be used for the growth of cells and the detection and measurement of a variety of biochemical processes and products using non-invasive techniques, that is techniques which do not compromise the integrity or viability of cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.