Methods and compositions for controlled polypeptide synthesis

US6632922B1 · kind B1 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Timothy J. Deming · Summerland, US
• Miaoer Yu · San Jose, US
• Scott Curtin · Berkeley, US
• Jungyeon Hwang · Goleta, US
• Michael D. Wyrsta · Lompoc, US

Key dates

• Filing date: May 10, 2000
• Grant date: Oct 14, 2003
• Expiry date: May 10, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions for the generation of polypeptides having varied material properties are disclosed herein. Methods include means for making self assembling amphiphilic block copolypeptides and related protocols adding oligo(ethyleneglycol) functionalized aninoacid-N-carboxyanhydrides (NCAs) to polyaminoacid chains. Additional methods include means of adding an end group to the carboxy terminus of a polyaminoacid chain by reacting an alloc-protected amino acid amide with a transition metal-donor ligand complex, thereby forming an amido-amidate metallacycle for use in further polymerization reactions. Novel compositions for use in peptide synthesis and design including five and six membered amido-containing metallacycles and block copolypeptides are also disclosed.