Method of increasing recovery of heterologous active enzymes produced in plants
US6632930B2 · kind B2 · utility
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Key dates
| Filing date | Jun 14, 2001 |
| Grant date | Oct 14, 2003 |
| Priority date | — |
| Expiry date | Jun 14, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y110/03002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of increasing recovery of active enzyme produced in a plant is provided where the enzyme requires a transitional metal cofactor for activation. The metal cofactor is supplied to the enzyme during plant development, during extraction, or after extraction. Recovery of active enzyme is also provided by incubating the extracted enzyme at a non-enzyme degrading temperature. Addition of a negative ion salt further improves active enzyme recovery. Optimum salt concentrations for recovery of laccase from plants using copper solutions is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.